Analysis of Antioxidant Constituents from and Evaluation of Their Blood-Brain Barrier Permeability.

Thunb., has been used to treat common cold, tonsillitis, and eczema. It is also a source of antioxidants. However, information regarding its antioxidative phytochemical composition is still incomplete and limited. In this present study, we initially determined DPPH radical scavenging activity of the extracts of fruits, twigs, and leaves. Among them, the twig extract exhibited a potential of antioxidant capacity. Based on antioxidant effect guided experiments, extraction condition using 80% EtOH was then optimized. DPPH and ABTS radical scavenging assays were also performed for fractions. The -butanol fraction showed the highest antioxidant effect. Using chromatographic methods, eight marker compounds (-) were further isolated. Their structures were determined by spectroscopic and mass data. Method validation was employed to quantitate contents of these eight marker compounds. Subsequently, the HPLC-DPPH method was used to evaluate the contribution of certain compounds to total antioxidant activity of the extract. Lastly, parallel artificial membrane permeability assay for blood-brain barrier (PAMPA-BBB) was applied to investigate brain-penetrable antioxidants from extract. As a result, compound (4,5-dicaffeoylquinic acid) showed significant antioxidant activity and penetration across the BBB via transcellular passive diffusion. Our findings suggested that compound can be used as a therapeutic potential candidate in natural product-based central nervous system (CNS) drug discovery.