The emergence of methicillin-resistant (MRSA) comprises a global threat to humans and animals. Here, we report and characterize the MRSA t304/ST6 variant which, to our knowledge, represents the first case found in bovine clinical mastitis. In general, the MRSA t304/ST6 variant is rarely described in livestock, contrary to humans where it is widely recognized. Phenotypic and genotypic resistance profiling showed that the bovine-MRSA t304/ST6 isolate expressed low susceptibility toward cefoxitin (MIC = 16 µg/mL) and carried the resistance gene in the SCC IVa. The bovine-MRSA t304/ST6 isolate carried a plasmid similar to that which has been frequently observed among human-MRSA t304/ST6 isolates in Denmark (GenBank accession no. NZ_CP047022). In addition, a Staphylococcus prophage 3 (ϕSA3) was detected, encoding an immune evasion cluster (IEC) of putative virulence genes associated with human host-specificity (, , and ). Taken together, these findings suggest that the MRSA t304/ST6 found in this study represents a recent host-jump event, with human to cow transmission. This study emphasizes the importance of and the need for performance of antimicrobial resistance surveillance among bovine mastitis pathogens, including and MRSA.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598757 | PMC |
| http://dx.doi.org/10.3390/antibiotics11101393 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Clonal dissemination of successful emerging clone -MRSA t304/ST6 among humans and hedgehogs in the Helsinki metropolitan area in Finland.
One Health
June 2023
Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Agnes Sjöberginkatu 2, PO Box 66, 00790, Helsinki, Finland.
Methicillin-resistant (MRSA) carrying gene (-MRSA) is frequently reported among European hedgehogs () due to co-evolutionary adaptation to dermatophyte infection in European hedgehogs. The occurrence of MRSA in European hedgehogs in Finland is unknown. Consequently, we investigated the occurrence of MRSA in wild hedgehogs from urban Helsinki metropolitan area in 2020-2021 and applied whole genome sequencing (WGS) to further characterize the studied isolates and compared them with human clinical MRSA isolates.
View Article and Find Full Text PDFFirst Finding of a Methicillin-Resistant (MRSA) t304/ST6 from Bovine Clinical Mastitis.
Antibiotics (Basel)
October 2022
Center for Diagnostics, Technical University of Denmark, Kemitorvet, 2800 Kgs. Lyngby, Denmark.
The emergence of methicillin-resistant (MRSA) comprises a global threat to humans and animals. Here, we report and characterize the MRSA t304/ST6 variant which, to our knowledge, represents the first case found in bovine clinical mastitis. In general, the MRSA t304/ST6 variant is rarely described in livestock, contrary to humans where it is widely recognized.
View Article and Find Full Text PDFA tale of two STs: molecular and clinical epidemiology of MRSA t304 in Norway 2008-2016.
Eur J Clin Microbiol Infect Dis
February 2022
The Norwegian MRSA Reference Laboratory, Department of Medical Microbiology, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
The purpose of this study was to investigate the epidemiological, molecular, and clinical characteristics of MRSA t304/ST8 and t304/ST6 in Norway from 2008 to 2016. Clinical and epidemiological data were collected for each case included in the study. Strains were characterized by PCR, spa typing, antimicrobial susceptibility testing, and whole genome sequencing.
View Article and Find Full Text PDFRepeated introduction and spread of the MRSA clone t304/ST6 in northern Europe.
Clin Microbiol Infect
February 2021
Department of Clinical Microbiology, MRSA Knowledge Centre, Hvidovre Hospital, Denmark; Institute of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.
Objectives: During the last decades several methicillin-resistant Staphylococcus aureus (MRSA) clones with the capability of global spread have emerged in the community. Here, we have investigated a large collection of clinical isolates belonging to MRSA clone t304/ST6, which has emerged in many European countries over the last years, in order to retrace its phylogeny and its spread.
Methods: We characterized 466 ST6 isolates from Denmark (n = 354), France (n = 10), Norway (n = 24), Sweden (n = 27) and the UK (n = 51).
Genotyping and antimicrobial resistance of Staphylococcus aureus isolates from dairy ruminants: differences in the distribution of clonal types between cattle and small ruminants.
Arch Microbiol
January 2020
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Staphylococcus aureus is part of the normal flora of animals, and represents one of the leading causes of contagious mastitis in dairy herds worldwide. Sixty-seven epidemiologically unrelated S. aureus isolates from nasal and mastitis milk samples of dairy-producing animals (32 cows, 25 sheep, and 10 goats) were characterized by antimicrobial susceptibility testing and spa typing followed by multilocus sequence typing (MLST) on representative isolates and SCCmec-typing on methicillin-resistant S.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!