Background: Actually, no data on the prevalence of plasmid colistin resistance in Tunisia are available among clinical bacteria.
Objectives: This study aimed to investigate the current epidemiology of colistin resistance and the spread of the gene in clinical Gram-negative bacteria (GNB) isolated from six Tunisian university hospitals.
Methods: A total of 836 GNB strains were inoculated on COL-R agar plates with selective screening agar for the isolation of GNB resistant to colistin. For the selected isolates, genes, beta-lactamases associated-resistance genes and molecular characterisation were screened by PCRs and sequencing.
Results: Colistin-resistance was detected in 5.02% (42/836) of the isolates and colistin-resistant isolates harboured an ESBL () and/or a carbapenemase (, ) encoding gene in 45.2% of the cases. The -1 gene was detected in four isolates (0.59%) causing urinary tract infections and all these isolates also contained the gene. The gene was detected in three isolates that also carried the IncY and IncFIB replicons. The genetic environment surrounding the -carrying plasmid indicated the presence of gene upstream -1 resistance marker with unusual missing of ISApl1 insertion sequence.
The Conclusions: This study reports the first description of the -1 gene among clinical isolates in Tunisia and provides an incentive to conduct routine colistin susceptibility testing in GNB clinical isolates.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9598684 | PMC |
| http://dx.doi.org/10.3390/antibiotics11101390 | DOI Listing |
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