AI Article Synopsis
- E7 protein from HPV is crucial for cancer development, making it a key target for treatment.
- Heat treatment can specifically destabilize the E7 mRNA oncotranscript complex, reducing its levels in HPV-infected cells.
- This method works by promoting the aggregation of IGF2BP1, which binds to the modified E7 mRNA, and ultimately helps reverse HPV-related cancer in lab settings.
Article Abstract
Human papillomavirus (HPV)-induced carcinogenesis critically depends on the viral early protein 7 (E7), making E7 an attractive therapeutic target. Here, we report that the E7 messenger RNA (mRNA)-containing oncotranscript complex can be selectively targeted by heat treatment. In HPV-infected cells, viral E7 mRNA is modified by N-methyladenosine (mA) and stabilized by IGF2BP1, a cellular mA reader. Heat treatment downregulates E7 mRNA and protein by destabilizing IGF2BP1 without the involvement of canonical heat-shock proteins and reverses HPV-associated carcinogenesis in vitro and in vivo. Mechanistically, heat treatment promotes IGF2BP1 aggregation only in the presence of mA-modified E7 mRNA to form distinct heat-induced mA E7 mRNA-IGF2BP1 granules, which are resolved by the ubiquitin-proteasome system. Collectively, our results not only show a mutual regulation between mA RNA and its reader but also provide a heat-treatment-based therapeutic strategy for HPV-associated malignancies by specifically downregulating E7 mRNA-IGF2BP1 oncogenic complex.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.celrep.2022.111546 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Selective inhibition of HSF1 expression in the heat shock pathway of keloid fibroblasts reduces excessive fibrosis in keloid.
Arch Dermatol Res
January 2025
Department of Plastic and Burn Surgery, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Wuhou District, Chengdu, 610041, Sichuan, China.
The stress response following burns may be a crucial factor in keloid formation, yet the underlying pathological mechanisms remain to be elucidated. This study initially investigated how heat shock factor 1 (HSF1) and heat shock proteins (HSPs) within the heat shock pathway influence keloid fibrosis, providing insights into the role of the heat shock response in keloid development. This study aims to further elucidate the role of the heat shock pathway in keloid fibrosis and investigate the specific function of HSF1 within this pathway.
View Article and Find Full Text PDFProtective Effects of Heat-Killed Lactobacilli against Plasma-Induced Neurotoxicity in Multiple Sclerosis.
Probiotics Antimicrob Proteins
January 2025
Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Heat-killed lactobacilli seem to have protective effects against oxidative stress and neurotoxicity. This study aimed to evaluate the antioxidant properties of specific heat-killed lactobacilli extracts and determine their neuroprotective effects against the neurotoxicity induced by blood plasma from people with multiple sclerosis (MS). The antioxidant activity of the three heat-killed lactobacilli was measured using the DPPH assay.
View Article and Find Full Text PDF[Climate change and ocular surface diseases].
Ophthalmologie
January 2025
Klinik für Augenheilkunde, Universitätsklinikum Düsseldorf, Moorenstr. 5, 40225, Düsseldorf, Deutschland.
Background: The ocular surface is directly exposed to environmental influences. Noxae that have already been identified for the ocular surface are heat, air dryness, pollutant gases, fine dust particles and ultraviolet radiation.
Methods: The current literature was used to investigate the relationship between frequent ocular surface diseases and various environmental factors and to analyze their development over the years.
Aqueous Proteomic and Metabolomic Profiles in Low-Energy vs High-Energy Femtosecond Laser-Assisted Cataract Surgery.
Invest Ophthalmol Vis Sci
January 2025
Singapore Eye Research Institute, Singapore.
Purpose: To investigate the aqueous proteomics and metabolomics in low-energy and high-energy femtosecond laser-assisted cataract surgery (FLACS).
Methods: In this prospective observational study, 72 patients were randomized to 3 groups: low-energy FLACS, high-energy FLACS, and conventional phacoemulsification (controls). Aqueous was collected after femtosecond laser treatment or at the beginning of surgery (controls).
Extreme temperatures elicit the accumulation of cardiac glycoside and their genin units in Calotropis procera by altering the expression of transcripts involved in its biosynthesis.
J Plant Res
January 2025
Botany Unit (SAIF & R), CSIR-CDRI, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, Uttar Pradesh, 226031, India.
Calotropis procera (Aiton) W.T. Aiton is a medicinal plant belonging to the family Apocynaceae as a core source of natural cardenolides.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!