AI Article Synopsis

  • A new immunosensor was developed for detecting ricin using both fluorescence and electrochemiluminescence (ECL) methods, incorporating a streptavidin-biotin signal amplification system.
  • * The sensor utilized CdSe/ZnS quantum dots, which enhanced detection sensitivity, showing a linear response to ricin concentrations from 0.1 to 100 ng/mL (fluorescence) and 0.01 to 100 ng/mL (ECL).
  • * With impressive limits of detection (81.7 pg/mL for fluorescence and 5.5 pg/mL for ECL), the sensor successfully detected ricin in complex samples and can potentially be adapted for other target proteins.*

Article Abstract

A sensitive dual-readout immunosensor for fluorescence and electrochemiluminescence (ECL) detection of ricin was established, which was combined with a streptavidin-biotin signal amplification system. CdSe/ZnS quantum dots with fine fluorescence and ECL properties were used as the dual-signal function probes of the sandwich immunocomplex. Under the optimum experimental conditions, the dual signal intensity increased significantly with the rise in ricin concentration. The fluorescence intensity of the senor exhibited a good liner relationship toward the ricin concentrations with 0.1~100 ng/mL and the limit of detection (LOD) was 81.7 pg/mL; taking ECL as the detection signal, the sensor showed a linear relationship with the ricin concentrations ranging from 0.01 ng/mL to 100 ng/mL and the LOD was 5.5 pg/mL. The constructed sensor with high sensitivity had been successfully applied to the detection of ricin in complex matrices with satisfactory recoveries. The proposed immunosensor model can be extended to the analysis and detection of others target proteins.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608998PMC
http://dx.doi.org/10.3390/toxins14100710DOI Listing

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