(1) Background: Microbial transglutaminases (MTGase) catalyze protein crosslink. This is useful in the food industry to improve gelation, water holding capacity, and emulsifying capacity during foodstuff manufacturing. The production of MTGase in wild-type strains renders low yield and high costs of downstream purification, limiting its industrial applications. (2) Methods: In this work, MTGase from BH072 (BaMTGase) has been heterologously expressed in , using the signal peptide Usp45 to direct the secretion of recombinant BaMTGase out of the cell for easier purification. (3) Results: In these conditions, MTGase was purified with a high yield (48.7 ± 0.2 mg/L) and high enzyme activity (28.6 ± 0.5 U/mg). Next, BaMTGase was tested for industrial applications. Recombinant BaMTGase and commercial MTGase were used for SPI solution crosslinking. BaMTGase formed a harder gel with higher water-holding capacity and a dense and smooth gel microstructure. (4) Conclusions: This work provides an attractive food-grade cell factory for the food industry and offers a suitable chassis for MTGase production.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9601987 | PMC |
| http://dx.doi.org/10.3390/gels8100674 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cyanobacterial phycoremediation: a sustainable approach to dairy wastewater management.
Environ Technol
January 2025
Botany Discipline, School of Biological Sciences and Biotechnology, Goa University, Goa, India.
The dairy industry is a significant sector within the food industries, known for its high-water consumption and consequent generation of dairy wastewater (DWW), which is rich in pollutants like Chemical Oxygen Demand (COD) and Biological Oxygen Demand (BOD). Improper disposal of DWW poses serious environmental challenges, including eutrophication and highlighting the need for sustainable biological treatment methods. This study investigates the potential of indigenous cyanobacterial strains , , , and for the bioremediation of DWW.
View Article and Find Full Text PDFEnzymatic asymmetric synthesis of l-phenylglycine by amino acid dehydrogenases has potential for industrial applications; however, this is hindered by their low catalytic efficiency toward high-concentration substrates. We identified and characterized a novel leucine dehydrogenase (LeuDH) with a high catalytic efficiency for benzoylformic acid via directed metagenomic approaches. Further, we obtained a triple-point mutant LeuDH-EER (D332E/G333E/L334R) with improved stability and catalytic efficiency through the rational design of distal loop 13.
View Article and Find Full Text PDFQuinoa Polysaccharides: Extraction, Purification, Structure, Functional Properties, and Applications in Food Science and Health.
Plant Foods Hum Nutr
January 2025
College of Food Science and Engineering, Ningbo University, Ningbo, 315832, PR China.
Quinoa polysaccharides have attracted significant research interest in recent years due to their diverse biological activities, including antiviral, anti-inflammatory, antioxidant, and immunoregulatory properties. These attributes align with the growing global demand for natural, functional food ingredients, positioning quinoa polysaccharides as a valuable resource in food science and technology. This review presents an overview of the various bioactivities of quinoa polysaccharides, critically evaluates the methods used for their extraction and purification, describes their structural characteristics, and discusses their practical applications across multiple areas within the food industry, including food additives, meat products, health foods, and innovative food packaging.
View Article and Find Full Text PDFMineral, Vitamin and Phytonutrients Content in Different Andean Potato Varieties Reintroduced in the North West Region, Argentina (NOA).
Plant Foods Hum Nutr
January 2025
Centro Interdisciplinario de Investigaciones en Tecnologías y Desarrollo Social para el NOA (CIITED), Facultad de Ingeniería, Universidad Nacional de Jujuy and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Jujuy, Argentina.
The mineral content, vitamin C, and chlorogenic acid were determined in 44 genotypes of Andean potatoes reintroduced in the Quebrada de Humahuaca, Jujuy, Argentina. This initiative aims to promote biodiversity and support local producers by determining the nutritional and functional properties of these genotypes. The genotypes from a collection in the Germplasm Bank of INTA Balcarce, were planted in sandy clay loam soils and harvested manually.
View Article and Find Full Text PDFCharacterization of a novel D-sorbitol dehydrogenase from Faunimonas pinastri A52C2.
Appl Microbiol Biotechnol
January 2025
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
The enzyme D-sorbitol dehydrogenase (SLDH) facilitates the conversion of D-sorbitol to L-sorbose. While current knowledge of this enzyme class predominantly centers on Gluconobacter oxydans, the catalytic properties of enzymes from alternative sources, particularly their substrate specificity and coenzyme dependency, remain ambiguous. In this investigation, we conducted BLASTp analysis and screened out a novel SLDH (Fpsldh) from Faunimonas pinastri A52C2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!