Genetics and Gene Editing Methods in and .

Cold Spring Harb Protoc

Department of Biology, University of Virginia, Charlottesville, Virginia 22903, USA

Published: June 2023

Our understanding of biological systems has for many years been heavily influenced by experimental approaches that exploit genetic methods. These include gain-of-function experiments that overexpress transgenes or ectopically express injected RNA and loss-of-function experiments that knock out genes or knock down RNAs. Here, we review how these methods have been applied in frogs and introduce a variety of protocols for genetic manipulation of and .

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http://dx.doi.org/10.1101/pdb.top107045DOI Listing

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