Production of biopolymer precursors beta-alanine and L-lactic acid from CO with metabolically versatile DSM 43205.

Hydrogen oxidizing autotrophic bacteria are promising hosts for conversion of CO into chemicals. In this work, we engineered the metabolically versatile lithoautotrophic bacterium strain DSM 43205 for synthesis of polymer precursors. Aspartate decarboxylase (panD) or lactate dehydrogenase (ldh) were expressed for beta-alanine or L-lactic acid production, respectively. The heterotrophic cultivations on glucose produced 25 mg L beta-alanine and 742 mg L L-lactic acid, while autotrophic cultivations with CO, H, and O resulted in the production of 1.8 mg L beta-alanine and 146 mg L L-lactic acid. Beta-alanine was also produced at 345 μg L from CO in electrobioreactors, where H and O were provided by water electrolysis. This work demonstrates that DSM 43205 can be engineered to produce chemicals from CO and provides a base for its further metabolic engineering.