sp. nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus .

A novel cellulose-degrading actinobacterium, designated strain NEAU-S10, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach. Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10 was a representative of and was closely related to NEAU-yn17 (99.2 %), SA152 (99.0 %), DSM 44231 (98.5 %) and NBRC 101911 (98.5 %). Physiological and chemotaxonomic characteristics of the strain further supported its affiliation to the genus . The whole-cell sugars contained galactose, ribose and mannose. The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-10(H). The major fatty acids were iso-C, C, anteiso-C, iso-C and iso-C. The genomic DNA G+C content was 71.8 mol%. The levels of digital DNA-DNA hybridization between isolate and NEAU-yn17, SA152 and DSM 44231 were 40.1 % (37.6-42.6 %), 38.soap8 % (36.3-41.3 %) and 44.8 % (42.2-47.3 %) and the ANI values between them were determined to be 90.2, 89.8 and 91.7 %, the results indicated that strain NEAU-S10 could be distinguished from its reference strains. The assembled genome sequence of strain NEAU-S10 was found to be 10 305 394 bp long. The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes. Genomic analysis and Congo red staining test indicated that strain NEAU-S10 had the potential to degrade cellulose. The genomic and phenotypic results indicate that strain NEAU-S10 represents a novel species of the genus , for which the name sp. nov. is proposed, with NEAU-S10 (=CCTCC AA 2020037=JCM 34800) as the type strain.