Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157/zg-ZUI40, zg-ZUI222/zg-ZUI199 and zg-ZUI188/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus . The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus , and a DNA G+C content range of 73.0-74.6 mol%. The major fatty acids detected in strain pairs zg-ZUI157/zg-ZUI40 and zg-ZUI 222/zg-ZUI199 were C, anteiso-C and anteiso A-C, and C, anteiso-C, anteiso A-C and anteiso-C in strain pair zg-ZUI188/zg-ZUI168. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species. MK-9(H) was the predominant quinone detected in strains zg-ZUI222 (87.4 %) and zg-ZUI188 (91.4 %), and MK-9(H) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157. The cell-wall sugars detected in the three novel species mainly contained rhamnose. The cell-wall peptidoglycan type of the three novel species was A4, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157 and zg-ZUI222, and l-Orn-d-Glu for strain zg-ZUI188. Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel species, for which the names sp. nov. (zg-ZUI157=GDMCC 1.2559=KCTC 49678), sp. nov. (zg-ZUI222=GDMCC 1.2501=KCTC 49675) and sp. nov. (zg-ZUI188=GDMCC 1.2563=KCTC 49674) are proposed.
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http://dx.doi.org/10.1099/ijsem.0.005533 | DOI Listing |
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