The goal of this work was to develop prototypes of open-hardware vitrification devices for sperm cryopreservation that can be integrated with existing straw platforms. The open-hardware Vitrification Device for French Straws (VD-FS) is low-cost, customizable, 3-D printable, standardized, and allows long-term sample storage and identification. The feasibility was shown for vitrifying and storing samples with multiple configurations. The results can be improved by design alternation and evaluation of various vitrification solutions. This is the first complete open-hardware vitrification device that can be integrated with existing French-straw storage systems, providing a foundation for future community-level modifications and improvements.
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http://dx.doi.org/10.1016/j.ohx.2022.e00366 | DOI Listing |
Eur J Med Res
December 2024
Department of Obstetrics and Gynecology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.
Background: Chronic salpingitis is one of the most common causes of female infertility. Luteal support is a critical step for embryo transfer. Here, we evaluated the effects of two luteal support regimens, intramuscular progesterone (IMP) and progesterone vaginal gel (VAG), on the pregnancy outcomes in patients with chronic salpingitis undergoing vitrified-warmed embryo transfer.
View Article and Find Full Text PDFCryobiology
December 2024
HemoCord - Umbilical Cord Blood Bank, Unisinos Technology Park, São Leopoldo, RS, Brazil.
One of the biggest challenges in studying vitrification protocols for small volumes of biological materials, especially the microdroplet vitrification protocol, is measuring the solidification rate, requiring equipment with a high level of technology, making it practically impossible to measure the degree of crystallization. An alternative is using mathematical models applied in computer simulations (CFD), helping to improve and develop new vitrification protocols. This study investigates the vitrification process utilizing the microdroplet method through experimental and numerical analysis.
View Article and Find Full Text PDFCryobiology
December 2024
Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, 55455, USA; Center for Advanced Technologies for the Preservation of Biological Systems (ATP-Bio), University of Minnesota, Minneapolis, MN, 55455, USA.
Cryopreservation of aquatic embryos or larvae is needed to help safeguard genetics from important wild and captive species, increase aquaculture output, and meet the global demand for protein. To this end, the development of a cryopreservation protocol for nauplius larvae of the commercially important aquaculture species Litopenaeus vannamei, or Pacific White Shrimp, was pursued. Toxicity screening was performed using multiple cryoprotective agents (CPA), and a multi-constituent CPA cocktail was developed to achieve reliable vitrification of shrimp larvae encapsulated in 1.
View Article and Find Full Text PDFRSC Adv
October 2024
J. Mike Walker '66 Department of Mechanical Engineering, Texas A&M University College Station TX 77843 USA
Aqueous vitrification (glass formation) processes are integral to modern cryopreservation, but experimental methods by which to study them are limited, particularly at the mL volume scales relevant to many biomedical applications. Here, we introduce an inexpensive custom optical platform, the isochoric vitrification cryo-macroscope (or "isovitriscope"), to supplement standard techniques with new qualitative and quantitative data streams. The platform consists of an LED light source, a isochoric (constant-volume) chamber with sapphire optical windows, and a camera, which can operate in two modes.
View Article and Find Full Text PDFLab Chip
November 2024
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Egg (oocyte) vitrification is the dominant method for preserving fertility for women of reproductive age. However, the method is typically performed by hand, requiring precise (∼0.1 to 10 μL) and time-sensitive (∼1 s) liquid exchange of cryoprotectants (CPA) around eggs as well as fine handling of eggs (∼100 μm) for immersion into liquid nitrogen (LN).
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