Colostrum known as "liquid gold" contains approximately 60-80% of whey proteins that can be a great source of bioactive peptide production. Therefore, this study aimed to perform a comparative antimicrobial evaluation of the bioactive peptide generated from C25, C6, and NCDC17 fermented colostrum whey. Peptide fractions 10 kDa, 5 kDa, and 3 kDa were isolated using their respective molecular weight cut-off membranes and antimicrobial activity was evaluated against diarrheagenic strains. The higher inhibition was shown by < 10 kDa peptide fractions from C25 fermented colostrum whey and the zone of inhibition was 15 ± 0.06 ( MTCC 723), 17 ± 0.04 ( MTCC 724), 18 ± 0.05 ( MTCC 725), and 17 ± 0.02 ( ATCC 25922). In addition, ST-1 and LT-1 genes of strains were also confirmed using PCR which is responsible for the diarrheagenic property. Further, the interaction of potent peptides against strains was also observed by scanning electron microscope. Hence, the significance of the present study emphasized that these bioactive peptides generated from fermented colostrum whey can be used as ingredients in functional food against diarrhoea.
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| http://dx.doi.org/10.1007/s13197-022-05606-x | DOI Listing |
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