Although mainstream partial nitrification-anammox (PN-A) is a highly efficient and sustainable wastewater treatment process, it is difficult to achieve and stabilize due to the competition among functional bacteria. In this study, achieving one-stage mainstream anammox via regulating bacteria community structure was studied in a lab-scale biological aerated filter (BAF). The results showed that high free ammonia with 89.57 mg/L, nitrite nitrogen (NO-N) competition between anammox bacteria (AnAOB) and nitrite oxidizing bacteria (NOB), and backwash regulated the bacteria community structure. After backwash, Candidatus Kuenenia became the dominant bacteria and the relative abundance increased to 5.56 %. In BAF, one-stage mainstream anammox with total nitrogen (TN) being lower than 15 mg/L in the effluent was achieved using lag-time of bacteria activity recovery caused by alternating operation of high and low ammonia nitrogen (NH-N), which have great potential applied in municipal wastewater treatment plants (MWWTPs).
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http://dx.doi.org/10.1016/j.biortech.2022.128091 | DOI Listing |
Bioresour Technol
January 2025
School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China.
Efficient nitrogen removal after organic capture is challenging through conventional nitrification-denitrification process. Two biofilm-based anoxic/oxic reactors, with a single intermittent zone (R1) or dual intermittent zones (R2), were compared in treating carbon-limited wastewater. Intermittent aeration integrated partial nitrification-anammox (PNA), partial denitrification-anammox (PDA), and denitrification, with anammox-related pathways contributing over 75% nitrogen removal in both reactors.
View Article and Find Full Text PDFBioresour Technol
January 2025
National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, PR China. Electronic address:
There is limited understanding of the granular partial denitrification/anammox (PD/A) microbiota and metabolic hierarchy specific to municipal wastewater treatment, particularly concerning the multi-mechanisms of functional differentiation and granulation tendencies under high-loading shocks. Therefore, this study utilized fragmented mature biofilm as the exclusive inoculum to rapidly establish a granular PD/A system. Following long-term feeding with municipal wastewater, PD/A process reached a total nitrogen removal efficiency of 97.
View Article and Find Full Text PDFWater Res X
May 2025
School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong 266237, China.
Anaerobic ammonia oxidation (anammox) which converts nitrite and ammonium to dinitrogen gas is an energy-efficient nitrogen removal process. One of the bottlenecks for anammox application in wastewater treatment is the stable supply of nitrite for anammox bacteria. Dissimilatory nitrate reduction to ammonium (DNRA) is a process that converts nitrate to nitrite and then to ammonium.
View Article and Find Full Text PDFChemosphere
January 2025
Biological Processes Laboratory (LPB), São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, São Paulo, 13563-120, Brazil.
Simultaneous partial nitrification, anammox, and denitrification (SNAD) process offers a promising method for the effective removal of carbon and nitrogen from wastewater. However, ensuring stability is a challenge. This study investigated operational parameters such as hydraulic retention time (HRT) and biomass retention to stabilize SNAD operation, transitioning from synthetic to anaerobically pre-treated municipal wastewater (APMW) in an upflow hybrid biofilm-granular reactor (UHR).
View Article and Find Full Text PDFEnviron Res
January 2025
School of Energy and Environmental Engineering, Hebei University of Technology, Tianjin, 300401, China.
Iron-loaded diatomite (Fe-DE) was developed as the innovative material to enhance anammox granular sludge (AnGS) and mainstream anammox performance. By adding Fe-DE with the Fe:DE ratio of 1:20 and the dosage of 3 g/L, the start-up period of mainstream anammox process was shortened from 29 d to 17 d and its nitrogen removal rate was increased from 0.234 kg N/(m·d) to 0.
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