AI Article Synopsis
- The study investigates how liver function is affected in areas with obstructed blood flow, using rat models to measure the uptake of indocyanine green (ICG) and the expression of liver-function-related mRNA.
- Twenty rats underwent surgery to ligate a hepatic vein, with subsequent assessments of ICG fluorescence intensity and mRNA expression levels at different time points post-surgery.
- Results showed significantly lower ICG uptake and mRNA levels in obstructed regions compared to non-obstructed areas, indicating impaired liver function, which could be important for estimating liver health after surgeries.
Article Abstract
Purpose: The liver function in outflow-obstructed regions is reportedly impaired; however, the functional decrease has not been quantitatively assessed. We therefore evaluated the uptake of indocyanine green (ICG) into hepatocytes and the mRNA expression associated with the liver function in outflow-obstructed regions using rat models.
Methods: A total of 20 rats with the ligation of the right median hepatic vein to induce outflow obstruction were studied. Five rats each were grouped by the time of re-laparotomy, and the fluorescence intensity (FI) values of ICG. The mRNA expression, including that of Albumin, Cytochrome P450 (Cyp) 1a2, Cyp3a1, Cyp7a1, and Gamma-glutamylcysteine synthetase, in outflow-obstructed (mRNA) and non-outflow-obstructed (mRNA) regions was assessed.
Results: Microscopic fluorescence imaging showed that the FI values were significantly lower in outflow-obstructed regions than in non-outflow-obstructed regions at 12 h, 24 h, and 3 days after ligation of the hepatic vein. The mRNA/mRNA ratios decreased to approximately 30% at 12 h after the outflow obstruction and increased to approximately 70-80% at 7 days.
Conclusions: The liver function in outflow-obstructed regions was impaired in terms of the uptake of ICG and the mRNA expression. Our findings may help estimate the postoperative functional remnant liver volume by considering the decrease in the liver function in outflow-obstructed regions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00595-022-02588-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessment of liver function-related mRNA expression and fluorescence imaging in outflow-obstructed regions in rats.
Surg Today
April 2023
Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
Article Synopsis
- The study investigates how liver function is affected in areas with obstructed blood flow, using rat models to measure the uptake of indocyanine green (ICG) and the expression of liver-function-related mRNA.
- Twenty rats underwent surgery to ligate a hepatic vein, with subsequent assessments of ICG fluorescence intensity and mRNA expression levels at different time points post-surgery.
- Results showed significantly lower ICG uptake and mRNA levels in obstructed regions compared to non-obstructed areas, indicating impaired liver function, which could be important for estimating liver health after surgeries.
Influence of outflow-obstructed liver volume and venous communication development: A three-dimensional volume study in living donors.
Liver Transpl
December 2017
Divisions of Hepato-Biliary-Pancreatic Surgery.
Living donor liver transplantation using the left liver graft with the middle hepatic vein (MHV) is a well-established procedure. Following such procedures, outflow obstruction occurs in remnant livers. However, the effects of the outflow-obstructed liver volume (LV ), with or without venous communication development, remain unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!