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Bioactive Metabolites of Lactiplantibacillus plantarum K014 Against Methicillin-Resistant Staphylococcus aureus ATCC43300 and In Vitro Evaluation of Its Antibacterial, Antioxidant and Anti-inflammatory Activities. | LitMetric

AI Article Synopsis

  • - The study investigates the antimicrobial effects of lactic acid bacteria (LAB) against methicillin-resistant Staphylococcus aureus (MRSA), focusing on the K014 strain from fermented vegetables, which demonstrated a 91.93% inhibition rate against MRSA.
  • - The K014 strain is identified as Lactiplantibacillus plantarum through 16S rRNA sequencing, and its genetic sequence has been submitted to the GenBank database.
  • - The cell-free supernatant from L. plantarum K014 shows heat and acid tolerance, produces bioactive metabolites with significant antioxidant (46.28%) and anti-inflammatory (43.66% for LOX assay) activities, indicating its potential for treating skin infections.

Article Abstract

This study aims to evaluate the effects of bioactive metabolites produced by lactic acid bacteria against methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300. A total of six lactic acid bacteria (LAB) were selected to evaluate the antimicrobial activity against MRSA ATCC 43300, a skin pathogen that is highly resistant to most antibiotics. The K014 isolate from a fermented vegetable recorded the highest inhibition against MRSA ATCC 43300 at 91.93 ± 0.36%. 16S rRNA sequencing revealed the K014 isolate is closely related to L. plantarum and the sequence was subsequently deposited in the GenBank database with an accession number of MW180960, named as Lactiplantibacillus plantarum K014. The cell-free supernatant (CFS) of L. plantarum K014 had tolerance to high temperature as well as acidic pH. The bioactive metabolites, such as hydrogen peroxide, lactic acid and hyaluronic acid, were produced by L. plantarum K014. Result from ABTS assay showed higher antioxidant activity (46.28%) as compared to that obtained by DPPH assay (2.97%). The CFS had showed anti-inflammatory activity for lipoxygenase (LOX) assay at 43.66%. The bioactive metabolites of L. plantarum K014 showed very promising potential to be used topical skin pathogens.

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Source
http://dx.doi.org/10.1007/s00284-022-03038-6DOI Listing

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