AI Article Synopsis
- Tetraspanin proteins play a crucial role in organizing cellular complexes on the membranes of most nucleated cells, but their specific functions in folliculogenesis and oogenesis in mammals are not fully understood.
- New research has highlighted the distribution of tetraspanins CD9, CD81, CD151, CD82, and CD63 at various stages of follicular development in cattle, indicating possible cooperation between CD9, CD63, and integrin alpha V in ovarian tissues.
- The study also uncovered unique localization patterns of tetraspanins in bovine oocytes and showed that the distribution of these proteins remains unchanged in vitrified oocytes, emphasizing their potential significance in oocyte development and fertilization processes in cattle.
Article Abstract
Tetraspanin proteins are mostly known as organizers of molecular complexes on cell membranes, widely expressed on the surface of most nucleated cells. Although tetraspanins participate in many physiological processes of mammals, including reproduction, their relevance to the processes of folliculogenesis and oogenesis has not yet been fully elucidated. We bring new information regarding the distribution of tetraspanins CD9, CD81, CD151, CD82, and CD63 at different stages of follicular development in cattle. The found distribution of tetraspanin CD9, CD63, and integrin alpha V in similar areas of ovarian tissue outlined their possible cooperation. We also describe yet-unknown distribution patterns of CD151, CD82, and CD63 on immature and mature bovine oocytes. The unique localization of tetraspanins CD63 and CD82 in the zona pellucida of bovine oocytes suggested their involvement in transzonal projections. Furthermore, we present an unchanged distribution pattern of the studied tetraspanins in vitrified mature bovine oocytes. The immunofluorescent analysis was supplemented by in silico data addressing tetraspanins expression in the ovarian cells and oocytes across several species. The obtained results suggest that in the study of the oocyte development and potentially the fertilization process of cattle, the role of tetraspanins and integrins should also be taken into account.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9922244 | PMC |
| http://dx.doi.org/10.1007/s00418-022-02155-4 | DOI Listing |
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