A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3098
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: Attempt to read property "Count" on bool

Filename: helpers/my_audit_helper.php

Line Number: 3100

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3100
Function: _error_handler

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

CLMP Promotes Leukocyte Migration Across Brain Barriers in Multiple Sclerosis. | LitMetric

CLMP Promotes Leukocyte Migration Across Brain Barriers in Multiple Sclerosis.

Neurol Neuroimmunol Neuroinflamm

From the Neuroimmunology Research Laboratory (A.P.F., S.Z., M.C., E.P., O.T., J.I.A., H.K., L.B., S.L., B., W.K., F.T., P.D., C.L., N.A., M.D.,P.D.A.P.), Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM); Department of Neurosciences (A.P.F., S.Z., M.C., E.P., F.T., C.L., N.A., M.D.,P.D.A.P.), Faculty of Medicine, Université de Montréal; Department of Microbiology (H.K.), Infectious Diseases and Immunology, Faculty of Medicine, Université de Montréal; Multiple Sclerosis Clinic (B., P.D., M.G., C.L., N.A., M.D.,P.D.A.P.), Division of Neurology, Centre Hospitalier de l'Université de Montréal (CHUM); Division of Neurosurgery (A.B., R.M.), Université de Montréal & CHUM; and Department of Pathology (R.C.), Université de Montréal & CHUM, Quebec, Canada.

Published: November 2022

AI Article Synopsis

  • - The study investigates the role of coxsackie and adenovirus receptor-like membrane protein (CLMP) in the migration of immune cells into the central nervous system (CNS) of patients with multiple sclerosis (MS), focusing on how it contributes to CNS damage.
  • - Researchers found that CLMP expression was significantly heightened in both the endothelial cells and immune cells of MS patients, particularly in active brain lesions, indicating its involvement in the inflammatory response associated with MS.
  • - Blocking CLMP with specific antibodies reduced immune cell migration across brain endothelial cells in laboratory tests, suggesting that targeting CLMP may offer a potential therapeutic approach for managing MS-related inflammation.

Article Abstract

Background And Objectives: In multiple sclerosis (MS), peripheral immune cells use various cell trafficking molecules to infiltrate the CNS where they cause damage.The objective of this study was to investigate the involvement of coxsackie and adenovirus receptor-like membrane protein (CLMP) in the migration of immune cells into the CNS of patients with MS.

Methods: Expression of CLMP was measured in primary cultures of human brain endothelial cells (HBECs) and human meningeal endothelial cells (HMECs), postmortem brain samples, and peripheral blood mononuclear cells (PBMCs) from patients with MS and controls by RNA sequencing, quantitative PCR, immunohistochemistry, and flow cytometry. In vitro migration assays using HBECs and HMECs were performed to evaluate the function of CLMP.

Results: Using bulk RNA sequencing of primary cultures of human brain and meningeal endothelial cells (ECs), we have identified CLMP as a new potential cell trafficking molecule upregulated in inflammatory conditions. We first confirmed the upregulation of CLMP at the protein level on TNFα-activated and IFNγ-activated primary cultures of human brain and meningeal ECs. In autopsy brain specimens from patients with MS, we demonstrated an overexpression of endothelial CLMP in active MS lesions when compared with normal control brain tissue. Flow cytometry of human PBMCs demonstrated an increased frequency of CLMP B lymphocytes and monocytes in patients with MS, when compared with that in healthy controls. The use of a blocking antibody against CLMP reduced the migration of immune cells across the human brain and meningeal ECs in vitro. Finally, we found CLMP immune cell infiltrates in the perivascular area of parenchymal lesions and in the meninges of patients with MS.

Discussion: Collectively, our data demonstrate that CLMP is an adhesion molecule used by immune cells to access the CNS during neuroinflammatory disorders such as MS. CLMP could represent a target for a new treatment of neuroinflammatory conditions.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9465835PMC
http://dx.doi.org/10.1212/NXI.0000000000200022DOI Listing

Publication Analysis

Top Keywords

immune cells
16
human brain
16
primary cultures
12
cultures human
12
endothelial cells
12
brain meningeal
12
clmp
11
brain
8
multiple sclerosis
8
cells
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: