Detecting aquatic pathogens with field-compatible dried qPCR assays.

J Microbiol Methods

Section for Aquatic Biology and Toxicology, Department of Biosciences, University of Oslo, P.O. Box 1066, Blindern 0316, Oslo, Norway; eDNA Solutions AB, Bjorkasgatan 16, SE-43131 Molndal, Gothenburg, Sweden. Electronic address:

Published: November 2022

Field-ready qPCR assays with extended shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf-life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying.

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Source
http://dx.doi.org/10.1016/j.mimet.2022.106594DOI Listing

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