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Biarsenical-based fluorescent labeling of metallothioneins as a method for ultrasensitive quantification of poly-Cys targets.
Anal Chim Acta
February 2025
Department of Chemical Biology, Faculty of Biotechnology, University of Wroclaw, Joliot-Curie 14a, 50-383, Wrocław, Poland. Electronic address:
Background: Mammalian metallothioneins (MTs) play a crucial role in maintaining Zn(II) and Cu(I) homeostasis, as well as regulating the cellular redox potential. They are involved in cancer resistance to cisplatin-related drugs and the sequestration of toxic metal ions. To investigate their participation in specific physiological and pathological processes, it is imperative to develop an analytical method for measuring changes in protein concentration both in vitro and in vivo.
View Article and Find Full Text PDFEffects of ECMF Isolated from Mining Areas on Water Status, Photosynthesis Capacity, and Lead Ion Transport of Under Pb Stress.
J Fungi (Basel)
November 2024
Institute of Applied Biotechnology, College of Agronomy and Life Sciences, Shanxi Datong University, Datong 037009, China.
Native ectomycorrhizal fungi (ECMF) are generally more effective than non-native ECMF in facilitating the phytoremediation of heavy metal (HM) ions from contaminated soils. This study aimed to investigate the contributions of four ECMF species-, , , and -that were isolated from mining areas to the growth, water status, photosynthesis, and metallothionein gene expression of exposed to varying concentrations of lead (Pb). The experiment lasted two months and involved .
View Article and Find Full Text PDFTwo birds with one stone: Alleviating copper toxicity and inhibiting its upward transport in non-host rice (Oryza sativa L.) by inoculation of Cu-resistant endophytes from the hyperaccumulator Commelina communis.
Environ Pollut
February 2025
School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, 410083, China. Electronic address:
Endophytic bacteria derived from metal hyperaccumulators have demonstrated potential for improving copper (Cu) remediation in host plants; however, their potential application in non-host crops remains unclear. In this study, endophytic bacteria isolated from Commelina communis growing in mining areas and their mitigation effects on Cu toxicity in non-host rice were comprehensively evaluated. Among the isolated endophytes, Bacillus sp.
View Article and Find Full Text PDF[Surface display and application of cadmium-binding protein CADR on the cell wall of ].
Sheng Wu Gong Cheng Xue Bao
October 2024
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China.
Algae has been proven to have the potential to be efficient biosorbents in the detection and remediation of heavy metal pollution such as cadmium in the environment. This study aims to enhance the cadmium adsorption capacity of by expressing the cadmium-binding protein CADR on the cell wall by the surface display technology. Firstly, the golden gate technique was employed to construct the transformation vector PET-X-, which anchored CADR to the cell wall with the cell wall protein GP1.
View Article and Find Full Text PDFRhizofungus Mitigates Heavy Metal Stress-Associated Damage in L.
Plants (Basel)
September 2024
Department of Botany, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
Industrial waste and sewage deposit heavy metals into the soil, where they can remain for long periods. Although there are several methods to manage heavy metals in agricultural soil, microorganisms present a promising and effective solution for their detoxification. We isolated a rhizofungus, (GenBank Acc.
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