AI Article Synopsis
- Umbelliferone (UM) has shown promise as an alternative to antibiotics by significantly inhibiting the virulence factors and quorum-sensing signals of a harmful aquatic pathogen.
- RNA sequencing revealed that UM downregulates genes involved in exotoxin production, secretory systems, and iron uptake, which contribute to its antivirulence effects.
- In animal studies, UM improved survival rates of infected grass carp, reduced bacterial loads, and lessened organ injuries, suggesting its potential as a treatment for such infections.
Article Abstract
is an important pathogen that mainly harms aquatic animals and has exhibited resistance to a variety of antibiotics. Here, to seek an effective alternative for antibiotics, the effects of umbelliferone (UM) at sub-MICs on virulence factors and the quorum-sensing system were studied. Subsequently, RNA sequencing was employed to explore the potential mechanisms for the antivirulence activity of umbelliferone. Meanwhile, the protective effect of umbelliferone on grass carp infected with was studied in vivo. Our results indicated that umbelliferone could significantly inhibit virulence such as hemolysis, biofilm formation, swimming and swarming motility, and their quorum-sensing signals AHL and AI-2. Transcriptomic analysis showed that umbelliferone downregulated expression levels of genes related to exotoxin, the secretory system (T2SS and T6SS), iron uptake, etc. Animal studies demonstrated that umbelliferone could significantly improve the survival of grass carps infected with , reduce the bacterial load in the various tissues, and ameliorate cardiac, splenic, and hepatopancreas injury. Collectively, umbelliferone can reduce the pathogenicity of and is a potential drug for treating infection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9569924 | PMC |
| http://dx.doi.org/10.3390/ijms231911119 | DOI Listing |
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