CTX-M-Producing Bacteria Isolated from a Highly Polluted River System in Portugal.

Int J Environ Res Public Health

Department of Life Sciences and CFE, University of Coimbra, 3000-456 Coimbra, Portugal.

Published: September 2022

resistant to third-generation cephalosporins are a great concern for public health, as these are first-line drugs to treat infections. The production of carbapenemases and extended spectrum beta-lactamases (ESBLs) and/or the overexpression of AmpC β-lactamases are the main mechanisms of resistance to these antibiotics. Among the ESBLs, CTX-M β-lactamases are the most prevalent worldwide. Our aims were to determine the prevalence of cefotaxime-resistant along a heavily polluted river and characterize carriers. River water was collected in 11 sites along the main course and tributaries, in two sampling moments. Water quality was evaluated and a collection of cefotaxime-resistant isolates was obtained. carriers were characterized regarding phylogenetic affiliation, clonality, antibiotic susceptibility, gene diversity, and context. Water presented very low quality in all sites. From 147 cefotaxime-resistant isolates, 46% carried and were affiliated with , , , and . Molecular typing revealed clonal isolates in different sites and over the two years, suggesting survival of the strains in the river or continuous pollution inputs from the same sources. Eight variants of were found, with being the most prevalent (52.5%). Sites with a lower water quality showed the highest resistance rates and prevalence of , suggesting that river water may embody human health risks.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9565674PMC
http://dx.doi.org/10.3390/ijerph191911858DOI Listing

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