AI Article Synopsis
- Induced pluripotent stem cells (iPSCs) can be created from somatic cells using specific factors (Oct4, Sox2, Klf4, c-Myc), and their reprogramming can be enhanced with small molecules like Licochalcone D (LCD).
- The study showed that LCD increased the efficiency of iPSC generation as the treated cells expressed pluripotency-related genes and successfully differentiated into all three germ layers.
- Additionally, LCD facilitated a key process called mesenchymal-to-epithelial transition (MET), where mesenchymal gene expression decreased and epithelial gene expression increased, indicating effective reprogramming.
Article Abstract
Induced pluripotent stem cells (iPSCs) can be generated from somatic cells using Oct4, Sox2, Klf4, and c-Myc (OSKM). Small molecules can enhance reprogramming. Licochalcone D (LCD), a flavonoid compound present mainly in the roots of , acts on known signaling pathways involved in transcriptional activity and signal transduction, including the PGC1-α and MAPK families. In this study, we demonstrated that LCD improved reprogramming efficiency. LCD-treated iPSCs (LCD-iPSCs) expressed pluripotency-related genes , and . Moreover, LCD-iPSCs differentiated into all three germ layers in vitro and formed chimeras. The mesenchymal-to-epithelial transition (MET) is critical for somatic cell reprogramming. We found that the expression levels of mesenchymal genes ( and ) decreased and those of epithelial genes (, and ) dramatically increased in OR-MEF (OG2/ROSA26) cells treated with LCD for 3 days, indicating that MET effectively occurred in LCD-treated OR-MEF cells. Thus, LCD enhanced the generation of iPSCs from somatic cells by promoting MET at the early stages of reprogramming.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668095 | PMC |
| http://dx.doi.org/10.4014/jmb.2208.08042 | DOI Listing |
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