[Effect of on Bacterial Stress Response and Intracellular Infection and Immunity].

Objective: To investigate the effect of ( ) on bacterial stress response and intracellular infection and immunity.

Methods: The target gene amplified from H37Rv genome was cloned to the vector and then transferred to ( ) to construct a recombinant strain. Stress response experiment and Raw264.7 mouse macrophage infection was carried out with , the recombinant strain, and , the vector strain. Tests were conducted to measure bacterial colony forming unit (CFU) and transcriptional levels of cytokines, including interleukin ( )-1β, -6, -10, -12 40, interferon ( )- , tumor necrosis factor ( )- , and inducible nitric oxide synthase ( ).

Results: The recombinant strain, , was constructed successfully. According to the findings of the stress response experiment, could indeed enhance bacterial survival under certain conditions of culture. Intracellular infection experiment demonstrated that enhanced bacterial survival in macrophages and influenced the transcriptional level of cytokines.

Conclusion: The genes from play a role in bacterial stress response and intracellular infection and immunity.