The behavior, transport, and positive regulation mechanism of ZnO nanoparticles in a plant-soil-microbe environment.

ZnO nanoparticles (ZnO NPs) have been widely used in several fields, and they have the potential to be a novel fertilizer to promote plant growth. For the effective use of ZnO NPs, it is necessary to understand their influence mechanisms and key interactions with the soil physical and biological environment. In this review, we summarize the fate and transport of ZnO NPs applied via soil treatment or foliar spray in plant-soil systems and discuss their positive regulation mechanisms in plants and microbes. The latest research shows that the formation, bioavailability, and location of ZnO NPs experience complicated changes during the transport in soil-plant systems and that this depends on many factors. ZnO NPs can improve plant photosynthesis, nutrient element uptake, enzyme activity, and the related gene expression as well as modulate carbon/nitrogen metabolism, secondary metabolites, and the antioxidant systems in plants. Several microbial groups related to plant growth, disease biocontrol, and nutrient cycling in soil can be altered with ZnO NP treatment. In this work, we present a systematic comparison between ZnO NP fertilizer and conventional zinc salt fertilizer. We also fill several knowledge gaps in current studies with the hope of providing guidance for future research.