The fatty acid synthesis (FAS) pathway is essential for bacterial survival. Acyl carrier proteins (ACPs), donors of acyl moieties, play a central role in FAS and are considered potential targets for the development of antibacterial agents. , a primary phytopathogenic bacterium, causes bacterial wilt in more than 200 plant species. The genome of contains five annotated genes, , , , , and . In this study, we characterized the five putative ACPs and confirmed that only AcpP1 is involved in FAS and is necessary for the growth of . We also found that AcpP2 and AcpP4 participate in the polyketide synthesis pathway. Unexpectedly, the disruption of four genes (, , , and ) allowed the mutant strain to grow as well as the wild-type strain, but attenuated the bacterium's pathogenicity in the host plant tomato, suggesting that these four ACPs contribute to the virulence of through mechanisms other than the FAS pathway.
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| http://dx.doi.org/10.3389/fmicb.2022.1014971 | DOI Listing |
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