The germination process is of central importance across the cultivated species involving several key enzymes for mobilization of stored food reserves. Pullulanase (PUL), a starch-debranching enzyme, plays an important role in mobilizing stored endosperm food reserves during germination. Pullulanase inhibitor (PULI) hinders PUL's activity through an unknown mechanism. Barley has one and two genes. During the time-dependent processes of seed germination, only expression shows an antagonistic relationship with that of . Our data have indicated that the expression of is modulated by SPL (Squamosa-promoter-binding Protein Like) transcription factors, known to be targeted by miR156. We show that the binding of recombinant HvSPL3 protein to the promoter occurs under reducing, but not under oxidizing conditions. Replacement of Cys residues with threonine in HvSPL3 abolishes the binding, indicating an essential role of the redox state in the expression of . Our findings may have important implications for the industrial use of starch.
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| http://dx.doi.org/10.1016/j.csbj.2022.09.027 | DOI Listing |
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