This study was designed to seek the phytochemical analysis, antioxidant, enzyme inhibition, and toxicity potentials of methanol and dichloromethane (DCM) extracts of aerial and root parts of . Total bioactive content, high-performance liquid chromatography-photodiode array detector (HPLC-PDA) polyphenolic quantification, and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis were utilized to evaluate the phytochemical composition. Antioxidant [including 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)], 2,2'-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power assay (FRAP), cupric reducing antioxidant capacity CUPRAC, phosphomolybdenum, and metal chelation assays] and enzyme inhibition [against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glucosidase, α-amylase, and tyrosinase] assays were carried out for biological evaluation. The cytotoxicity was tested against MCF-7 and MDA-MB-231 breast cell lines. The root-methanol extract contained the highest levels of phenolics (37.69 mg gallic acid equivalent/g extract) and flavonoids (83.0 mg quercetin equivalent/g extract) contents, and was also the most active for DPPH (50.04 mg Trolox equivalent/g extract) and CUPRAC (139.96 mg Trolox equivalent /g extract) antioxidant assays. Likewise, the aerial-methanol extract exhibited maximum activity for ABTS (94.05 mg Trolox equivalent/g extract) and FRAP (64.23 mg Trolox equivalent/g extract) assays. The aerial-DCM extract was noted to be a convincing cholinesterase (AChE; 4.01 and BChE; 4.28 mg galantamine equivalent/g extract), and α-glucosidase inhibitor (1.92 mmol acarbose equivalent/g extract). All of the extracts exhibited weak to modest toxicity against the tested cell lines. A considerable quantities of gallic acid, catechin, 4-OH benzoic acid, syringic acid, vanillic acid, 3-OH-4-MeO benzaldehyde, epicatechin, -coumaric acid, rutin, naringenin, and carvacrol were quantified HPLC-PDA analysis. UHPLC-MS analysis of methanolic extracts from roots and aerial parts revealed the tentative identification of important phytoconstituents such as polyphenols, saponins, flavonoids, and glycoside derivatives. To conclude, this plant could be considered a promising source of origin for bioactive compounds with several therapeutic uses.
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http://dx.doi.org/10.3389/fpls.2022.988352 | DOI Listing |
Mar Drugs
December 2024
Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Republic of Korea.
Sargahydroquinoic acid (SHQA), a bioactive compound found in certain species, exhibits significant health benefits. This study optimized the extraction of SHQA from using response surface methodology (RSM) and evaluated its antioxidant effects through in vitro and in vivo assays. A Box-Behnken design (BBD) was effectively employed to investigate the effects of incubation temperature, time, and ethanol concentration on SHQA yield, achieving a high coefficient of determination (R = 0.
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December 2024
Chemistry Department, College of Education Salahaddin University Erbil Iraq.
Evaluation of of the family Boraginaceae during previous investigations determined numerous therapeutic potentials against inflammatory-related diseases. The present study evaluates the phytochemical, acute toxicity, and hepatoprotective effects of methanolic extracts of (MEAL) against thioacetamide (TAA)-induced liver injury in rats. The phytochemical profiling of MEAL followed a Folin-Ciocalteu and 10% AlCl3 procedure using a spectrophotometer.
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December 2024
Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Alexandria, 21934, Egypt.
Methanolic extract from was investigated for its phytochemical content, antioxidant, and antimicrobial properties against phytopathogenic fungi and bacteria. Phytochemical analysis revealed the presence of saponin, tannins, and alkaloids with 1.25%, 18.
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November 2024
Université de Technologie de Compiègne, ESCOM, TIMR (Integrated Transformations of Renewable Matter), Centre de Recherche Royallieu-CS 60319, 60203 Compiègne CEDEX, France.
In this study, three extraction methods of bioactive compounds from the brown algae were ranked using the fuzzy weighting system in two stages, ranking between different conditions and choosing the optimal conditions for each extraction method separately. The inputs included extraction yield (EY), antioxidant activity (DPPH), total flavonoid content (TFC), total phenolic content (TPC), total phlorotannin content (TPhC), time, temperature, power, and cost. The top ranks of the first phase output included: Maceration Extraction (ME) with a score of 52.
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November 2024
Department of Analytical Chemistry, Nutrition & Food Sciences, University of Alicante, ES-03690 San Vicente del Raspeig, Alicante, Spain.
Date seeds, often discarded during industrial processing, are an underexploited by-product rich in polyphenols with significant antioxidant potential. This study explores the extraction of polyphenols from date seeds using microwave-assisted extraction (MAE) with an organic solvent. The extraction process was optimized using response surface methodology (RSM), focusing on extraction time, ethanol concentration, and temperature.
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