AI Article Synopsis
- This is a scientific method that uses pig eyes to study how certain enzymes can change the gel-like substance in the eye into a liquid.
- The process includes careful cutting and handling to make sure there’s little damage and that it can be done on a larger scale.
- This method can help researchers understand how this eye change happens as people get older or when they have certain diseases.
Article Abstract
This protocol describes an ex vivo model to quantify enzymatic effects on vitreous liquefaction using porcine eyes in a transwell-plate system via induced syneresis. It provides a standardized dissection process and performs critical steps for gel-liquid separation with high precision, minimal tissue loss, and scalability. The protocol can be applied to other studies investigating vitreous liquefaction or gelatinous tissue analysis and can also serve to study vitreous liquefaction in vivo as it may occur during aging or disease progression.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9562426 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2022.101754 | DOI Listing |
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