AI Article Synopsis
- There's a significant challenge in glioma research due to the absence of in vitro models that mimic the characteristics of glioma cells found in live tissues.
- Researchers have developed a 3D-engineered scaffold using advanced polymerization techniques to create a more accurate cell culture system with patient-derived glioma cells.
- This 3D model allows for improved observation of cell behavior and structure compared to traditional 2D cultures, making it a valuable tool for studying brain cancer mechanics and testing potential treatments.
Article Abstract
A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D-engineered scaffold, fabricated by two-photon polymerization, is developed as a cell culture model system to study patient-derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy, and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient-derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D-engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks is observed for cells cultured on the scaffolds. The 3D-engineered glioma scaffolds therefore represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies.
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| http://dx.doi.org/10.1002/smll.202204485 | DOI Listing |
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