AI Article Synopsis
- The text reviews various electrochemical methods, including vesicle impact electrochemical cytometry and single cell amperometry, for studying neurotransmitter storage in cellular vesicles.
- Protocols are provided for measuring vesicle content and catecholamine release, allowing assessment of the fraction of neurotransmitter released during exocytosis.
- The study emphasizes the importance of these methods in understanding neuronal function and their potential impact on cognitive processes.
Article Abstract
We review the methods of vesicle impact electrochemical cytometry, intracellular impact electrochemical cytometry, and single cell amperometry and their application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluate factors related to neuronal function at the cellular level and implications in, for example, cognition.
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| http://dx.doi.org/10.1007/978-1-0716-2671-9_16 | DOI Listing |
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