AI Article Synopsis
- The regulation of calcium (Ca) fluxes through organelle membranes is crucial for both local and global calcium signaling that triggers exocytosis in chromaffin cells.
- Intraorganellar calcium homeostasis also influences organelle-specific functions, including mitochondrial energy production, secretory granule maturation, and endoplasmic reticulum stress response.
- This chapter discusses techniques for studying calcium homeostasis in living chromaffin cells using engineered aequorins specifically targeted to mitochondria, endoplasmic reticulum, and secretory vesicles.
Article Abstract
The molecular mechanisms that mediate and regulate calcium (Ca) fluxes through the membranes of intracellular organelles play a key role in the generation and shaping of the local and global cytosolic Ca signals triggering the process of regulated exocytosis in chromaffin cells. Beyond that role, intraorganellar Ca homeostasis also regulates organelle-specific processes such as oxidative phosphorylation in mitochondria, maturation of secretory granules, or stress in the endoplasmic reticulum. In this chapter, we describe current methods to study mitochondrial, endoplasmic reticulum, and secretory vesicle calcium homeostasis in living chromaffin cells using engineered targeted aequorins.
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