AI Article Synopsis
- The study investigates how rhCNB affects immune response in mice with cyclophosphamide-induced immunodeficiency through the TLR/MAPK signaling pathway.
- The research involved creating three groups of mice: a control group, an immunosuppressed group, and a rhCNB treatment group, followed by various blood analyses and tissue observations.
- Results indicated that rhCNB treatment resulted in improved immune function by enhancing TLR/MAPK signaling and balancing Th1/Th2 cytokines, contrary to the decline seen in the immunosuppressed model group.
Article Abstract
Background: This study aims to explore the immunomodulatory effect of rhCNB on mice with cyclophosphamide (CTX)-induced immunodeficiency through TLR/MAPK pathway.
Methods: BALB/c mice were randomly divided into three groups: a negative control group, an immunosuppression model group, and a rhCNB treatment group. Tail vein injection of cyclophosphamide (40 mg/kg) was used to establish a mouse immunosuppression model. Intraperitoneal injection of rhCNB (20 mg/kg) was administered to the treatment group, whereas equal quantities of normal saline were given to the control group and model group. Perform peripheral blood routine of CD4, CD8, and CD19 lymphocyte subsets and peripheral blood Th1/Th2 cell subsets 24 hours after the last administration. RT-PCR was used to detect mRNA levels of TLR, P38, JNK, T-bet, and GATA, the spleen immune organ index was measured, and the histopathological status of the spleen and thymus was observed.
Results: The results showed that compared with the control group, WBC, PLT, LYM, NEU, immune organ index, CD4/CD8 and CD19 subgroup ratio, and peripheral blood Th1/Th2 cell subgroups decreased in the model group. The mRNA levels of TLR, P38, JNK, T-bet, and GATA decreased compared with the model group, while they increased in the treatment group.
Conclusions: rhCNB has an immunomodulatory effect by regulating the expression of Th1/Th2 cytokine balance through the TLR/MAPK signaling pathway and promoting the differentiation and proliferation of lymphocytes, thereby improving the immune function.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9532092 | PMC |
| http://dx.doi.org/10.1155/2022/4891399 | DOI Listing |
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