Green chemistry is a useful tool for producing valuable chemicals from biomass. However, extracted compounds need to be tested for safety and efficacy before their use in humans. Here we investigate the chemical composition and biological effects of a leaves . supercritical carbon dioxide (SCCO) extract. Terpenes represented the main extract fraction, with Germacrene D (11.18%), delta-cadinene (10.54%), and alpha-pinene (8.7%) the most abundant molecules. Challenged with endothelial cells (ECs), increasing extract concentrations failed to affect cell proliferation or promote cell toxicity. ROS assessment in unstressed and HO-treated ECs revealed an extract dose-dependent antioxidant activity. Exposition of HO-treated ECs to increasing extract concentrations dose-dependently counteracted HO-induced cell impairments. The extract significantly counteracted fetal calf serum-induced ECs migration. For the first time, we report that a SCCO extract obtained from PL leaves is safe on ECs and may be a useful source of valuable compounds with vasculoprotective properties.
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| http://dx.doi.org/10.1080/14786419.2022.2130304 | DOI Listing |
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