Short and medium chain acylcarnitines have been used for the diagnosis of various fatty acid oxidation and organic acid disorders. This report presents a multiplex and quantitative analysis of acylcarnitines using MALDI-TOF MS based on a parylene matrix chip. The parylene matrix chip was fabricated by the deposition of a nanoporous film of parylene on an organic matrix array, which reduced the number of mass peaks from the organic matrix in the low / range. Quantitative analysis was possible using the parylene matrix chip because of the formation of nano-sized sample crystals on the nanoporous parylene film. Seven acylcarnitines were quantitatively analyzed using the chip; the method detection range included the cut-off values for metabolic disorders. The seven acylcarnitines of different concentrations were simultaneously detected using the parylene matrix chip and the interference from the mixed carnitines was estimated. Real L-carnitine (C0) samples were analyzed using serial dilution, and the recoveries were calculated by comparisons with a standard curve.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/d2an01199a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High-Throughput Quantitative Analysis of Amino Acids in Freeze-Dried Drops Using Time-of-Flight Secondary Ion Mass Spectrometry.
Anal Chem
March 2024
Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Republic of Korea.
Article Synopsis
- Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a valuable tool for analyzing biological samples, but its effectiveness is limited by the need for an ultrahigh vacuum environment and matrix effects that complicate measurements.
- The study introduces a new platform that allows for quick and efficient quantitative analysis of amino acids using a microarray with freeze-dried solution drops that form matrix deposits.
- By mixing amino acid solutions with stable isotope-labeled phenylalanine and utilizing the microarray setup, the researchers achieved a lower quantitation limit of less than 10 μM for amino acids in their samples.
Scaling down the dimensions of a Fabry-Perot polymer film acoustic sensor for photoacoustic endoscopy.
J Biomed Opt
January 2024
University of Michigan, Department of Mechanical Engineering, Ann Arbor, Michigan, United States.
Significance: A Fabry-Perot (FP) polymer film sensor can be used to detect acoustic waves in a photoacoustic endoscope (PAE) if the dimensions can be adequately scaled down in size. Current FP sensors have limitations in size, sensitivity, and array configurability.
Aim: We aim to characterize and demonstrate the imaging performance of a miniature FP sensor to evaluate the effects of reduced size and finite dimensions.
Synthesis of Wrinkle-Free Metallic Thin Films in Polymer by Interfacial Instability Suppression with Nanoparticles.
Nanomaterials (Basel)
March 2023
College of Engineering, Texas A&M University-Corpus Christi, Corpus Christi, TX 78412, USA.
Synthesis of a smooth conductive film over an elastomer is vital to the development of flexible optics and wearable electronics, but applications are hindered by wrinkles and cracks in the film. To date, a large-scale wrinkle-free film in an elastomer has yet to be achieved. We present a robust method to fabricate wrinkle-free, stress-free, and optically smooth thin film in elastomer.
View Article and Find Full Text PDFOptimization of Parylene C and Parylene N thin films for use in cellular co-culture and tissue barrier models.
Sci Rep
March 2023
Department of Microsystems Engineering, Rochester Institute of Technology, 77 Lomb Memorial Drive, Rochester, NY, 14623, USA.
Parylene has been used widely used as a coating on medical devices. It has also been used to fabricate thin films and porous membranes upon which to grow cells. Porous membranes are integral components of in vitro tissue barrier and co-culture models, and their interaction with cells and tissues affects the performance and physiological relevance of these model systems.
View Article and Find Full Text PDFSimultaneous analysis of acylcarnitines using MALDI-TOF mass spectrometry based on a parylene matrix chip.
Analyst
November 2022
Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-gu, Seoul, Korea.
Short and medium chain acylcarnitines have been used for the diagnosis of various fatty acid oxidation and organic acid disorders. This report presents a multiplex and quantitative analysis of acylcarnitines using MALDI-TOF MS based on a parylene matrix chip. The parylene matrix chip was fabricated by the deposition of a nanoporous film of parylene on an organic matrix array, which reduced the number of mass peaks from the organic matrix in the low / range.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!