AI Article Synopsis
- The study investigates MotB, a protein encoded by an early gene of bacteriophage T4, which may play a role in manipulating the host's genetic expression to aid the virus's takeover.
- Using advanced methods like RNA-seq and mass spectrometry, the researchers analyzed how overexpressing MotB affects RNA and protein levels during T4 infection in a specific bacterial strain.
- Findings reveal that MotB has a predicted two-domain structure and influences host gene expression, particularly downregulating several host tRNAs, which may optimize conditions for T4 replication during infection.
Article Abstract
Background: Although many bacteriophage T4 early genes are nonessential with unknown functions, they are believed to aid in the takeover of the host. Understanding the functions of these genes could be helpful to develop novel antibacterial strategies. MotB, encoded by a previously uncharacterized T4 early gene, is a DNA-binding protein that compacts the host nucleoid and alters host gene expression.
Methods: MotB structure was predicted by AlphaFold 2. RNA-seq and mass spectrometry (MS) analyses were performed to determine RNA and protein changes when was overexpressed in BL21(DE3) ±5 min T4 infection.
Results: MotB structure is predicted to be a two-domain protein with N-terminal Kyprides-Onzonis-Woese and C-terminal oligonucleotide/oligosaccharide-fold domains. In B, overexpression during infection does not affect T4 RNAs, but affects the expression of host genes, including the downregulation of 21 of the 84 chargeable host tRNAs. Many of these tRNAs are used less frequently by T4 or have a counterpart encoded within the T4 genome. The MS analyses indicate that the levels of multiple T4 proteins are changed by overexpression.
Conclusion: Our results suggest that in this B host, is involved in establishing a more favorable tRNA pool for the phage during infection.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527043 | PMC |
| http://dx.doi.org/10.1089/phage.2022.0023 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel Antibacterial 4-Piperazinylquinoline Hybrid Derivatives Against : Design, Synthesis, and In Vitro and In Silico Insights.
Molecules
December 2024
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze, 90128 Palermo, Italy.
Molecular hybridization, which consists of the combination of two or more pharmacophores into a single molecule, is an innovative approach in drug design to afford new chemical entities with enhanced biological activity. In the present study, this strategy was pursued to develop a new series of 6,7-dimethoxy-4-piperazinylquinoline-3-carbonitrile derivatives (-) with potential antibiotic activity by combining the quinoline, the piperazinyl, and the benzoylamino moieties, three recurrent frameworks in antimicrobial research. Initial in silico evaluations were conducted on the designed compounds, highlighting favorable ADMET and drug-likeness properties, which were synthesized through a multistep strategy, isolated, and fully characterized.
View Article and Find Full Text PDFRNAi-mediated downregulation of endogenous 4-coumarate: CoA ligase activity in Sorghum bicolor to alter the lignin content, which augmented the carbohydrate content and growth.
Planta
January 2025
Advanced Laboratory for Plant Genetic Engineering, Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur, India.
This study seeks to improve the biomass extractability of Sorghum bicolor by targeting a critical enzyme, 4CL, through metabolic engineering of the lignin biosynthetic pathway at the post-transcriptional level. Sorghum bicolor L., a significant forage crop, offers a potential source of carbohydrate components for biofuel production.
View Article and Find Full Text PDFSINE retrotransposons import polyadenylation signals to 3'UTRs in dog (Canis familiaris).
Mob DNA
January 2025
Department of Biology, La Sierra University, Riverside, CA, USA.
Background: Messenger RNA 3' untranslated regions (3'UTRs) control many aspects of gene expression and determine where the transcript will terminate. The polyadenylation signal (PAS) AAUAAA (AATAAA in DNA) is a key regulator of transcript termination and this hexamer, or a similar sequence, is very frequently found within 30 bp of 3'UTR ends. Short interspersed element (SINE) retrotransposons are found throughout genomes in high copy numbers.
View Article and Find Full Text PDFNFATc1 fosters allergic contact dermatitis responses by enhancing the induction of IL-17-producing CD8 cells.
J Invest Dermatol
December 2024
Department of Dermatology, Venereology and Allergology, University Hospital Würzburg, 97080 Würzburg, Germany. Electronic address:
A plethora of data supports a major role of CD4 and CD8 T lymphocytes for the initiation, progression and maintenance of allergic contact dermatitis (ACD). However, in-depth understanding of the molecular mechanisms is still limited. NFATc1 plays an essential role in T cell activation.
View Article and Find Full Text PDFThe genetic code is not universal.
Biosystems
January 2025
The Ionian School, Early Evolution of Life Department, Genetic Code and tRNA Origin Laboratory, Via Roma 19, 67030, Alfedena, L'Aquila, Italy. Electronic address:
Recently, a new genetic code with 62 sense codons, coding for 21 amino acids, and only 2 termination codons has been identified in archaea. The authors argue that the appearance of this variant of the genetic code is due to the relatively recent and complete recoding of all UAG stop codons to codons encoding for pyrrolysine. I re-evaluate this discovery by presenting arguments that favour the early, i.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!