AI Article Synopsis
- The text discusses a common genital mycoplasma that can lead to reproductive issues and negative pregnancy outcomes in both men and women.
- Traditional diagnostic methods, like pathogen culture and PCR, are slow and require specialized equipment and trained personnel.
- A new detection method is introduced that combines catalytic hairpin assembly with a lateral flow immunoassay, allowing results in just 30 minutes using simple tools like a water bath and fluorescence reader.
Article Abstract
is a common genital mycoplasma in men and women, which can cause reproductive tract infection and infertility, and is also related to adverse pregnancy outcomes and neonatal diseases. Pathogen culture and polymerase chain reaction (PCR) are the main methods for the diagnosis of . However, pathogen culture takes too long, and PCR requires professional personnel and sophisticated instruments. Here, we report a simple, convenient, sensitive, and specific detection method, which combines catalytic hairpin assembly with a lateral flow immunoassay strip. Only a water bath and a fluorescence reader are needed to detect the results in 30 min. We can realize the point-of-care testing of by this method. To verify this method, we selected 10 clinical samples for testing, and the test results were exactly the same as the clinical report.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9520684 | PMC |
| http://dx.doi.org/10.1021/acsomega.2c02457 | DOI Listing |
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