AI Article Synopsis
- This study investigates how hypoxia affects the expression of long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in human placenta-derived mesenchymal stem cells (hP-MSCs), focusing on their role in regulating cell behavior under low oxygen conditions.
- Researchers created a hypoxic environment using a multigas incubator and discovered that it significantly enhances the proliferation of hP-MSCs, identifying 289 lncRNAs and 240 mRNAs with altered expression levels.
- The study highlights that a specific upregulated lncRNA can boost hP-MSC proliferation by activating the PI3K/AKT signaling pathway and increasing the expression of proteins related
Article Abstract
Background: The effect of hypoxia on mesenchymal stem cells (MSCs) is an emerging topic in MSC biology. Although long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) are reported to play a critical role in regulating the biological characteristics of MSCs, their specific expression and co-expression profiles in human placenta-derived MSCs (hP-MSCs) under hypoxia and the underlying mech anisms of lncRNAs in hP-MSC biology are unknown.
Aim: To reveal the specific expression profiles of lncRNAs in hP-MSCs under hypoxia and initially explored the possible mechanism of lncRNAs on hP-MSC biology.
Methods: Here, we used a multigas incubator (92.5% N, 5% CO, and 2.5% O) to mimic the hypoxia condition and observed that hypoxic culture significantly promoted the proliferation potential of hP-MSCs. RNA sequencing technology was applied to identify the exact expression profiles of lncRNAs and mRNAs under hypoxia.
Results: We identified 289 differentially expressed lncRNAs and 240 differentially expressed mRNAs between the hypoxia and normoxia groups. Among them, the lncRNA was upregulated under hypoxia, which was also validated by reverse transcription-polymerase chain reaction. was confirmed to affect hP-MSC proliferation rates using a knockdown model. overexpression could significantly enhance the proliferation capacity of hP-MSCs, activate the PI3K/AKT pathway, and upregulate the expression of cell cycle-related proteins.
Conclusion: Our results revealed the specific expression characteristics of lncRNAs and mRNAs in hypoxia-cultured hP-MSCs and that lncRNA can promote hP-MSC proliferation through the PI3K/AKT pathway.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9516465 | PMC |
| http://dx.doi.org/10.4252/wjsc.v14.i9.714 | DOI Listing |
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