2S albumin g13 polypeptide, less related to Fag e 2, can be eliminated in common buckwheat ( Moench) seeds.

2S albumin (g11, g13, g14, and g28) is an important allergen in common buckwheat (). g13 is hydrophobic, rare in seeds, and may show distinct allergenicity from the others; therefore, we tried to eliminate this protein. Phylogenetic and property distance analyses indicated g13 is less related to g14 (Fag e 2) than g11/g28 is related to g14, particularly in the second domain containing the II and III α-helices. A null allele with a 531 bp insertion in the coding region was found for at an allele frequency of 2 % in natural populations of common buckwheat. The allele homozygote accumulated no g13 protein. A BLAST search for the 531 bp insertion suggested the insert-like sequence resided frequently in the buckwheat genome, including the self-incompatibility responsible gene in . The insert-like sequence could, therefore, help in producing hypoallergenic cultivars, and expand the genetic diversity of buckwheat.