Evaluating the expression pattern of oxacillinases (OXA) carbapenemases is essential to understand the prevalence and spread of carbapenem resistance . The aim of the study is to evaluate the presence of OXA carbapenemase genes and IS upstream to these genes in carbapenem-resistant clinical isolates. isolated from clinical samples were phenotypically identified and antibiotics sensitivity was performed. Multiplex polymerase chain reaction (PCR) was used to detect OXA51-like gene, OXA carbapenemases genes (OXA-23-like, OXA-24-like, and OXA-58-like), and IS in carbapenem-resistant isolates. Out of 55 isolates, 54 were confirmed as by PCR. -like gene was observed in 51 isolates of and none of the isolates showed the presence of -like and -like genes. Presence of IS upstream to OXA-23-like gene, OXA-51-like gene, and both OXA-51-like/OXA-23-like genes was observed in 51, 7, and 4 isolates, respectively. The genetic pattern of carbapenem-resistant isolated in this study was unique, which should be factored for clinical protocols to manage infections caused by emerging resistant strains of .
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| http://dx.doi.org/10.1055/s-0041-1732809 | DOI Listing |
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