Whole Genome Sequence Analysis of Bacteriophage P2.

Pol J Microbiol

Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.

Published: September 2022

Phage P2 was isolated from failed fermentation broth carried out by IMAU10120. A previous study in our laboratory showed that this phage belonged to the family. In this study, this phage's genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608156PMC
http://dx.doi.org/10.33073/pjm-2022-037DOI Listing

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