Evaluation and validation of reference genes in for real-time quantitative PCR.

Biotechniques

College of Life Sciences, Zhengzhou Normal University, Zhengzhou, 450044, People's Republic of China.

Published: October 2022

For all organs at all stages, , and can be selected as reference genes. For organs of the vegetative stage, and can be chosen for analysis of normalized gene expression. For the bud stage, and can be used for analysis of gene expression. For the full blossom stage, , and can be introduced into relative gene expression analysis. For vegetative organs, and can be used as reference genes. For reproductive organs, , and can be used as a reference for data processing. gene expression is more consistent when , and are used as reference genes.

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Source
http://dx.doi.org/10.2144/btn-2022-0025DOI Listing

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