sp. nov., isolated from water in Baiyang Lake.

A novel Gram-stain-negative, short-rod-shaped, orange-pigmented bacterial strain, designated L-1-4 w-11, was isolated from Baiyang Lake in China. The strain grew at 15-35 °C (optimum 30 °C) and pH 7-8 (optimum pH 7) in TSA medium. The predominant polar lipids of strain L-1-4 w-11 were sphingoglycolipid, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and three unidentified lipids; the major cellular fatty acids were Cω6 and summed feature 8 (Cω7 and/or Cω6); and the major respiratory quinone was ubiquinone 10 (Q-10). Strain L-1-4 w-11 showed the highest 16S rRNA gene sequence similarity to JCM 15438 (98.3 %) and GDMCC 1.657 (98.0 %). The draft genome size of strain L-1-4 w-11 was 3.3 Mbp, and the G+C content was 67.8 mol%. Digital DNA-DNA hydridization and average nucleotide identity values between the genome sequences of strain L-1-4 w-11 and GDMCC 1.657 (76.9 and 21.0 %), JCM 15438 (76.0 and 19.9 %) and CGMCC 1.12825 (72.8 and 19.6 %) were far below the thresholds for prokaryotic conspecific assignment. With the evidence from the phylogenetic, chemotaxonomic and genotypic analyses, we propose that strain L-1-4 w-11 represents a novel species with the name sp. nov. The type strain is L-1-4 w-11 (=CGMCC 1.13572=JCM 33962).