spelaea () is a free-living amoeba, proved to cause like keratitis with quite difficult treatment. This study aimed to evaluate the amoebicidal effect of () on trophozoites and cysts both in vitro and in vivo using Chinchilla rabbits as an experimental model of this type of keratitis. Chemical constituents of the aqueous extract of were identified using Liquid Chromatography-mass Spectrometry (LC-MS). In vitro showed a significant inhibitory effect on trophozoites and cysts compared to the reference drug, chlorhexidine (CHX) as well as the non-treated control ( < 0.05) with statistically different effectiveness in terms of treatment durations and concentrations. No cytotoxic effect of on corneal cell line was found even at high concentrations (32 mg/ml) using agar diffusion method. The in vivo results confirmed the efficacy of where the extract enhanced keratitis healing with complete resolution of corneal ulcers in 80% of the infected animals by day 14 (post infection)pi compared to 70% recovery with CHX after 20 treatment days. The therapeutic effect was also approved at histological, immune-histochemical, and parasitological levels. Our findings support the potential use of as an effective agent against keratitis.
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| http://dx.doi.org/10.1016/j.jsps.2022.06.005 | DOI Listing |
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