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| http://dx.doi.org/10.1002/ctm2.1059 | DOI Listing |
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Timescale and genetic linkage explain the variable impact of defense systems on horizontal gene transfer.
Genome Res
January 2025
Centro de Astrobiología (CAB), CSIC-INTA, Institute for Biocomputation and Physics of Complex Systems (BIFI), University of Zaragoza
Prokaryotes have evolved a wide repertoire of defense systems to prevent invasion by mobile genetic elements (MGE). However, because MGE are vehicles for the exchange of beneficial accessory genes, defense systems could consequently impede rapid adaptation in microbial populations. Here, we study how defense systems impact horizontal gene transfer (HGT) in the short and long terms.
View Article and Find Full Text PDFCRISPR/Cas Systems as Diagnostic and Potential Therapeutic Tools for Enterohemorrhagic .
Arch Immunol Ther Exp (Warsz)
January 2025
Department of Animal, Veterinary, and Food Science, University of Idaho, Moscow, Idaho, USA.
Following its discovery as an adaptive immune system in prokaryotes, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) system has been developed into a multifaceted genome editing tool. This review compiles findings aimed at implementation of this technology for selective elimination or attenuation of enterohemorrhagic (EHEC). EHEC are important zoonotic foodborne pathogens that cause hemorrhagic colitis and can progress to the life-threatening hemolytic uremic syndrome (HUS).
View Article and Find Full Text PDFViability and Motility of Under Elevated Martian Salt Stresses.
Life (Basel)
November 2024
Astrobiology Group, Center of Astronomy and Astrophysics, Technical University Berlin, 10623 Berlin, Germany.
This study investigates the effects of three Martian-relevant salts-sodium chlorate, sodium perchlorate, and sodium chloride-on the viability and motility of , a model organism for understanding microbial responses to environmental stress. These salts are abundant on Mars and play a crucial role in forming brines, one of the few sources of stable liquid water on the planet. We analyze the survivability under different salt concentrations using colony plating.
View Article and Find Full Text PDFApplication of the SpCas9 inhibitor BRD0539 for CRISPR/Cas9-based genetic tools in .
Biosci Microbiota Food Health
September 2024
Department of Agricultural Chemistry, Graduate School of Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Although the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system has been extensively developed since its discovery for eukaryotic and prokaryotic genome editing and other genetic manipulations, there are still areas warranting improvement, especially regarding bacteria. In this study, BRD0539, a small-molecule inhibitor of Cas9 (SpCas9), was used to suppress the activity of the nuclease during genetic modification of , as well as to regulate CRISPR interference (CRISPRi). First, we developed and validated a CRISPR-SpCas9 system targeting the gene of .
View Article and Find Full Text PDFComparative genomics analyses of Actinobacteriota identify Golgi phosphoprotein 3 (GPP34) as a widespread ancient protein family associated with sponge symbiosis.
Microbiome
January 2025
Department of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Haifa, Israel.
Background: Sponges harbor microbial communities that play crucial roles in host health and ecology. However, the genetic adaptations that enable these symbiotic microorganisms to thrive within the sponge environment are still being elucidated. To understand these genetic adaptations, we conducted a comparative genomics analysis on 350 genomes of Actinobacteriota, a phylum commonly associated with sponges.
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