AI Article Synopsis
- Concern over food and feed toxicosis, particularly from Aflatoxin-M1 (AF-M1) in milk, drives the need for effective detection methods globally.
- The study presents an electrochemical microfluidic biosensor that utilizes aptamers and graphene quantum dot-gold nanoparticles to enhance sensitivity and conductivity for detecting AF-M1 in milk samples.
- The sensor demonstrated a linear detection range of 100 pM to 2 nM and a limit of detection at 0.3 nM, while also evaluating its selectivity against similar-sized interfering molecules.
Article Abstract
Toxicosis through food and feed has remained a point of concern to food sectors across the globe. Although strict regulations have been implemented in developed and developing countries, their detection in food matrices is an evolving science. This study focuses on the development and fabrication of an electrochemical microfluidic biosensor that deploys aptamers to detect trace concentrations of Aflatoxin-M1 (AF-M1) in milk samples. The use of graphene quantum dot composite with Au nanoparticles anchors the aptamer to the sensor surface and improves its signal conductivity. The screen-printed carbon electrode modified with graphene quantum dot-gold nanoparticles is placed between two polydimethylsiloxane layers to promote portability and improve mixing effects. Differential pulse voltammograms indicated that the linear range of the sensor was between 100 pM and 2 nM making the limit of detection 0.3 nM. Interfering molecules of similar size were analyzed, to evaluate sensor selectivity.
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| http://dx.doi.org/10.1016/j.foodchem.2022.134302 | DOI Listing |
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