AI Article Synopsis
- A feasibility study was conducted to test the effectiveness of 16S rRNA gene analysis via the MinION sequencer for identifying bacteria in ocular infections.
- The study assessed DNA from various ocular samples, utilizing both 16S rRNA gene sequencing and conventional culture methods for comparison.
- Results showed that the MinION sequencing was quick and accurate, identifying more diverse bacterial species than traditional methods in some cases, enabling faster diagnosis of ocular infections.
Article Abstract
Objective: We conducted a feasibility study to verify the effectiveness of 16S ribosomal RNA (rRNA) gene analysis using the nanopore sequencer MinION for identifying causative bacteria in several types of ocular infections.
Methods And Analysis: Four cases of corneal ulcers, one case of endophthalmitis and one case of a conjunctival abscess were included in this study. DNA was extracted from corneal scraping, vitreous samples and secretions from the conjunctival abscess. We conducted 16S rRNA gene amplicon sequencing using MinION and metagenomic DNA analysis. The efficacy of bacterial identification was verified by comparing the conventional culture method with smear observations.
Results: 16S rRNA gene sequencing analysis with MinION identified the causative organisms promptly with high accuracy in approximately 4 hours, from ophthalmic specimens. The results of the conventional culture method and 16S rRNA gene sequencing were consistent in all cases. In four of the six cases, a greater variety of organisms was found in the 16S rRNA gene analysis than in bacterial culture.
Conclusion: Using our workflow, 16S rRNA gene analysis using MinION enabled rapid and accurate identification possible in various kinds of bacterial ocular infections.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131114 | PMC |
| http://dx.doi.org/10.1136/bmjophth-2021-000910 | DOI Listing |
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