Phage therapy (PT), as a method to treat bacterial infections, needs identification of bacteriophages targeting specific pathogenic host. , a Gram-positive coccus resident in the human gastrointestinal tract, may become pathogenic in hospitalized patients showing acquired resistance to vancomycin and thus representing a possible target for PT. We isolated four phages that infect and characterized them by host range screening, transmission electron microscopy, and genome sequencing. We also identified and three-dimensional modeled a new hyaluronidase enzyme. The four phages belong to family: three (namely vB_EfaS_TV51, vB_EfaS_TV54, and vB_EfaS_TV217) and one (vB_EfaS_TV16). All of them are compatible with lytic cycle. vB_EfaS_TV16 moreover presents a gene encoding for a hyaluronidase enzyme. The identified phages show features suggesting their useful application in PT, particularly the that may be of enhanced relevance in PT because of its potential biofilm-digestion capability.
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http://dx.doi.org/10.1089/phage.2021.0003 | DOI Listing |
3 Biotech
January 2025
School of Engineering and Sciences, Tecnologico de Monterrey, Campus Querétaro, Av. Epigmenio Gonzalez, No. 500 Fracc. San Pablo, 76130 Querétaro, Mexico.
Skin aging is characterized by progressive loss of functionality and regenerative potential of the skin, resulting in the appearance of wrinkles, irregular pigmentation, a decrease of elasticity, dryness, and rough texture. Damage to the skin caused by oxidative stress could substantially be slowed down by the use of phytochemicals that function as natural antioxidants. Although phytochemicals have immense potential as anti-aging medicines, their effectiveness as therapeutic agents is restricted by their poor solubility, biodistribution, stability, and hydrophilicity.
View Article and Find Full Text PDFFASEB J
January 2025
Division of Gastroenterology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.
Cancer metastasis is the leading cause of cancer-related deaths, making early detection and the prevention of metastatic progression critical research priorities. Recent studies have expanded our understanding of CEMIP (KIAA1199, HYBID), revealing its involvement in cancer metastasis and its potential role in slowing cancer progression. CEMIP plays critical roles in several stages of cancer metastasis: First, CEMIP promotes cancer cell proliferation to maintain cell heterogeneity before the metastasis process.
View Article and Find Full Text PDFJ Fungi (Basel)
December 2024
Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Hyaluronidases have been a subject of great interest in medical and cosmeceutical applications. Previously, our group demonstrated that the venom glands of contain hyaluronidase enzymes (VesT2s), and heterologous expression of the corresponding gene () in systems results in inclusion bodies, necessitating functional folding using urea. Here, we report the successful heterologous expression of VesT2a in the expression system, with gene construction achieved using Golden.
View Article and Find Full Text PDFMar Drugs
December 2024
Interdisciplinary Center of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal.
The natural products industry is gaining increasing interest, not only due to modern lifestyles becoming more aware of environmental and sustainability issues but also because of the loss of efficacy and undesirable side effects of synthetic ingredients. This pioneering study provides a comprehensive comparison between extracts obtained from wild and commercial samples of Linnaeus, highlighting their multifaceted benefits in cosmetic applications. The antiaging potential of acetone (70 and 90%) and ethanol 60% extracts from wild and commercial samples of , focusing on their application in cosmetics, was explored.
View Article and Find Full Text PDFBioresour Bioprocess
December 2024
Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Lihu Avenue No. 1800, Wuxi, 214122, People's Republic of China.
Hyaluronan (HA), a natural high molecular weight polysaccharide, has extensive applications in cosmetology and medical treatment. Hyaluronan-degrading enzymes (Hyals) act as molecular scissors that cleave HA by breaking the glucosidic linkage. Hyals are present in diverse organisms, including vertebrates, invertebrates and microorganisms, and play momentous roles in biological processes.
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