Analysis of Four New Phages and Modeling of a Hyaluronidase Catalytic Domain from .

Phage (New Rochelle)

Computational Biology Unit, Department of Biology, University of Bari, Bari, Italy.

Published: September 2021

Phage therapy (PT), as a method to treat bacterial infections, needs identification of bacteriophages targeting specific pathogenic host. , a Gram-positive coccus resident in the human gastrointestinal tract, may become pathogenic in hospitalized patients showing acquired resistance to vancomycin and thus representing a possible target for PT. We isolated four phages that infect and characterized them by host range screening, transmission electron microscopy, and genome sequencing. We also identified and three-dimensional modeled a new hyaluronidase enzyme. The four phages belong to family: three (namely vB_EfaS_TV51, vB_EfaS_TV54, and vB_EfaS_TV217) and one (vB_EfaS_TV16). All of them are compatible with lytic cycle. vB_EfaS_TV16 moreover presents a gene encoding for a hyaluronidase enzyme. The identified phages show features suggesting their useful application in PT, particularly the that may be of enhanced relevance in PT because of its potential biofilm-digestion capability.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041502PMC
http://dx.doi.org/10.1089/phage.2021.0003DOI Listing

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