Draft Genome Sequence of the Reference Strain of the Korean Medicinal Mushroom KMCC03342.

Mycobiology

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.

Published: August 2022

is a wood-decay brown rot fungus belonging to the family Polyporaceae. While the fungus grows, the sclerotium body of the strain, dubbed Bokryeong in Korean, is formed around the roots of conifer trees. The dried sclerotium has been widely used as a key component of many medicinal recipes in East Asia. strain KMCC03342 is the reference strain registered and maintained by the Korea Seed and Variety Service for commercial uses. Here, we present the first draft genome sequence of KMCC03342 using a hybrid assembly technique combining both short- and long-read sequences. The genome has a total length of 55.5 Mb comprised of 343 contigs with N50 of 332 kb and 95.8% BUSCO completeness. The GC ratio was 52.2%. We predicted 14,296 protein-coding gene models based on gene prediction and evidence-based annotation procedure using RNAseq data. The annotated genome was predicted to have 19 terpene biosynthesis gene clusters, which was the same number as the previously sequenced strain MD-104 genome but higher than Chinese strains. The genome sequence and the predicted gene clusters allow us to study biosynthetic pathways for the active ingredients of .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467534PMC
http://dx.doi.org/10.1080/12298093.2022.2109874DOI Listing

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