Objective: This study was to examine the anti-inflammatory effect of sappanone A on interleukin- (IL-) 1-stimulated osteoarthritis (OA) chondrocytes.
Methods: Chondrocytes were pretreated with sappanone A for 2 h before subsequent IL-1 stimulation. The mRNA expression levels of iNOs, COX-2, aggrecan, and collagen-II were measured with qRT-PCR. The levels of TNF-, IL-6, IL-8, MMP-3, and MMP-13 were determined by ELISA. The protein levels of iNOs, COX-2, ADAMTS-4, ADAMTS-5, aggrecan, collagen-II, p-p65, p65, IB, Nrf2, and HO-1 were assessed by Western blot.
Results: Sappanone A inhibited the IL-1-stimulated production of NO, PGE2, iNOS, COX-2, TNF-, IL-6, and IL-8 in OA chondrocytes. In addition, sappanone A suppressed the expression of MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5 in IL-1-stimulated OA chondrocytes. The degradation of ECM components was reversed by sappanone A. Sappanone A prevented NF-B activation while enhanced Nrf2/HO-1 activation in IL-1-treated chondrocytes.
Conclusion: Sappanone A may be a potent therapeutic agent for OA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9507726 | PMC |
| http://dx.doi.org/10.1155/2022/2380879 | DOI Listing |
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