Enzyme-Linked Oligonucleotide Assay (ELONA).

Methods Mol Biol

Aptus Biotech SL, Madrid, Spain.

Published: September 2022

Aptamers are single-stranded oligonucleotides able to recognize a target with high affinity and specificity. Aptamers are used in different diagnostics applications, highlighting, among all, variations of the traditional enzyme-linked immunosorbent assay (ELISA). In this chapter, we show the procedures for the development of two types of indirect ELONA: a sandwich ELONA and a direct ELONA coupled to either real-time quantitative PCR as a direct and sensitive readout.

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http://dx.doi.org/10.1007/978-1-0716-2695-5_18DOI Listing

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